8 × 15 k human microrna-specific microarray v2 platform (Agilent technologies)
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8 × 15 K Human Microrna Specific Microarray V2 Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Identifying microRNA/mRNA dysregulations in ovarian cancer
Journal: BMC Research Notes
doi: 10.1186/1756-0500-5-164
Figure Legend Snippet: MicroRNAs that separate tumour from normal . The intersection of three separate statistical tests yielded 127 microRNA that more significantly differentiate tumour from normal samples. The tests included an unpaired T-test ( p < 0.05) with a Benjamini Hochberg FDR multiple testing correction and an unpaired Mann-Whitney test ( p < 0.05) with a Benjamini Hochberg FDR multiple testing correction from within GeneSpring GX 11.0, and a Student's t-test ( p < 0.05). Data for these 127 microRNA were subsequently used for further clustering of the samples and SNR analysis to uncover the top microRNA which differentiate tumour and normal samples.
Techniques Used: MANN-WHITNEY
Figure Legend Snippet: PCA on robust microRNA data reveals distinct separation . PCA was executed using only the expression data from the 127 microRNA represented in Figure 1. A clear separation between tumour and normal samples validates the effectiveness of the methods used to generate this microRNA list.
Techniques Used: Expressing
Figure Legend Snippet: A separate cluster for normal samples implies significance of 18 microRNA . We K-means clustered (k = 3) all samples using microRNA expression data exclusively from the 18 microRNA in Table 1. The results are consistent with our expectation that these 18 microRNA best separate the cancerous samples from normal ovarian tissue.
Techniques Used: Expressing
Figure Legend Snippet: Anti-correlated and positively correlated mRNA/microRNA pairs in the tumour and/or the normal samples
Techniques Used:
Figure Legend Snippet: Anti-correlated and positively correlated mRNA/microRNA pairs in the tumour and/or the normal samples
Techniques Used:
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